Read the Rylo camera preview See It See it $60 at Best Buy I thought this might be a mistake, but, no, the weirdly named HP Laptop 15t Value is indeed quite the value at this price. Specs include an Intel Core i7 processor, 12GB of RAM, a 256GB solid-state drive and a 15.6-inch display. However, I strongly recommend paying an extra $50 to upgrade that display to FHD (1,920×1,080), because you’re not likely to be happy with the native 1,366×768 resolution. CNET may get a commission from retail offers. $155 at Google Express HP Laptop 15t Value: $520 (save $780) Angela Lang/CNET DJI’s answer to GoPro’s action cameras is rugged little model that’s shockproof, dustproof and waterproof down to 11 meters. It normally runs $350, but this deal drops it to $261 when you apply promo code 19LABOR10 at checkout. 0 Sarah Tew/CNET An Echo Dot makes a fine match for any Fire edition TV, because you can use the latter to say things like, “Alexa, turn on the TV.” Right now, the 24-inch Insignia Fire TV Edition starts at just $100, while the 32-inch Toshiba Fire TV Editions is on sale for $130. Just add any Fire TV Edition to your cart, then add a third-gen Echo Dot, and presto: The latter is free. What’s cooler: A snapshot of a firework exploding in front of you, or full 360-degree video of all the fireworks and all the reactions to seeing them? Oooh, ahhh, indeed. At $250, the compact Rylo dual-lens camera is selling for its lowest price yet. And for an extra $50, you can get the bundle that includes the waterproof housing.This deal runs through Sept. 3; it usually costs $500. Apple AirPods with Wireless Charging Case: $155 (save $45) Sarah Tew/CNET $999 Comments Review • iPhone XS review, updated: A few luxury upgrades over the XR $6 at Tidal TVs Speakers Mobile Accessories Cameras Laptops Automobiles Smart Speakers & Displays $261 at Daily Steals via Google Express See at Turo See It $59 at eBay Turo $90 at Daily Steals via Google Express Google Nest Hub: $59 (save $70) Lenovo Smart Clock: $59.99 (save $20) $520 at HP $999 Read Google Home Hub review $999 JBL Soundgear wearable speaker: $90 (save $160) Post a comment Free Echo Dot with an Insignia or Toshiba TV (save $50) Sarah Tew/CNET Rylo Chris Monroe/CNET Tags Recently updated to include digital-photo-frame capabilities, the Lenovo Smart Clock brings Google Assistant goodness to your nightstand. It’s a little smaller than the Amazon Echo Show 5, but also a full $30 less (and tied with Prime Day pricing) during this Best Buy Labor Day sale. Mentioned Above Apple iPhone XS (64GB, space gray) Share your voice Best Buy Rylo 5.8K 360 Video Camera: $250 (save $250) Sprint Lenovo 130-15AST 15.6-inch laptop: $210 (save $90) $299 at Amazon See It Sarah Tew/CNET The problem with most entry-level laptops: They come with mechanical hard drives. That makes for a mighty slow Windows experience. This Lenovo model features a 128GB solid-state drive, so it should be pretty quick to boot and load software, even with its basic processor. Plus, it has a DVD-burner! That’s not something you see in many modern laptops, especially at this price. Formerly known as the Google Home Hub, Google’s Nest Hub packs a wealth of Google Assistant goodness into a 7-inch screen. At $59, this is within a buck of the best price we’ve seen. It lists for $129 and sells elsewhere in the $89-to-$99 range.This is one item of many available as part of eBay’s Labor Day Sale (which, at this writing, doesn’t specifically mention Labor Day, but that’s how it was pitched to us). Though not technically a Labor Day sale, it’s happening during Labor Day sale season — and it’s too good not to share. Nationwide Distributors, via Google Express, has just about the best AirPods deal we’ve seen (when you apply promo code ZBEDWZ at checkout). This is for the second-gen AirPods with the wireless charging case. Can’t imagine these will last long at this price, so if you’re interested, act fast. Other Labor Day sales you should check out Best Buy: In addition to some pretty solid MacBook deals that have been running for about a week already, Best Buy is offering up to 40% off major appliances like washers, dryers and stoves. There are also gift cards available with the purchase of select appliances. See it at Best BuyDell: Through Aug. 28, Dell is offering an extra 12% off various laptops, desktops and electronics. And check back starting Aug. 29 for a big batch of Labor Day doorbusters. See it at DellGlassesUSA: Aug. 29 – Sept. 3 only, you can save 65% on all frames with promo code labor65. See it at GlassesUSALenovo: The tech company is offering a large assortment of deals and doorbusters through Labor Day, with the promise of up to 56% off certain items — including, at this writing, the IdeaPad 730S laptop for $700 (save $300).See it at LenovoLensabl: Want to keep the frames you already love and paid for? Lensabl lets you mail them in for new lenses, based on your prescription. From now through Sept. 2 only, you can save 20% on the blue light-blocking lens option with promo code BLOCKBLUE. See it at LensablSears: Between now and Sept. 7, you can save up to 40% on appliances (plus an additional 10% if you shop online), up to 60% on mattresses, up to 50% on Craftsman products and more. The store is also offering some fairly hefty cashback bonuses. See it at SearsNote: This post was published previously and is continuously updated with new information.CNET’s Cheapskate scours the web for great deals on tech products and much more. For the latest deals and updates, follow the Cheapskate on Facebook and Twitter. Questions about the Cheapskate blog? Find the answers on our FAQ page, and find more great buys on the CNET Deals page. Tidal 3-month family subscription: $5.99 (save $54) Amazon expanded its fleet to 50 aircraft. Digital First Media/Orange Count More people are using Amazon Prime than ever. The online retail giant surpassed 100 million Prime subscribers earlier this year, and Cyber Monday 2018 was the site’s biggest shopping day yet. So how will Amazon keep up with this demand? Apparently by buying more airplanes.On Friday, Amazon announced that it was expanding its fleet to 50 aircraft (up from 40). Amazon says this is to support the increasing number of Prime subscribers who expect free two-day delivery. (On top of that, Amazon customers bought 2 billion items with one-day delivery this year.)By adding 10 more aircraft, Amazon is expanding its fleet by 25 percent — a sizable increase. But this fleet is still dwarfed by other shipping companies. UPS owns 247 aircraft (and leases more during November and December), while FedEx owns over 650 (ranking it among the world’s largest airlines).If Amazon is to continue offering free-two day shipping to its millions of Prime subscribers, it better have the arsenal to back it up. Boost Mobile Read DJI Osmo Action preview See at Amazon Use promo code 19LABOR10 to get an unusually good deal on JBL’s interesting hybrid product — not quite headphones, and not quite a traditional speaker, but something you wear like neckphones to listen to music on the go. Read Lenovo Smart Clock review Turo is kind of like Uber meets Airbnb: You borrow someone’s car, but you do all the driving. I’ve used it many times and found it a great alternative to traditional car-rental services — in part because you get to choose exactly the vehicle you want (not just, say, “midsize”) and in part because you can often do pickup and dropoff right outside baggage claim.Between now and Sept. 1, the first 300 people to check out can get $30 off any Turo rental with promo code LDW30. Turo: Save $30 on any car rental Apple iPhone XS Preview • iPhone XS is the new $1,000 iPhone X $210 at Best Buy Best laptops for college students: We’ve got an affordable laptop for every student. Best live TV streaming services: Ditch your cable company but keep the live channels and DVR. Internet Tech Industry The Cheapskate $999 Amazon Prime Amazon,I’m shocked — shocked! — to learn that stores are turning Labor Day into an excuse to sell stuff. Wait — no, I’m not. As much as I respect the original intent of the holiday (which became official back in 1894), to most of us, it’s just a bonus day off — one that’s blissfully tacked onto a weekend. So, yeah, stores; go ahead, run your sales. I’m listening. Perhaps unsurprisingly, Labor Day doesn’t bring out bargains to compete with the likes of Black Friday (which will be here before you know it), but there are definitely some sales worth your time.For example:We’ve rounded up the best Labor Day mattress deals.We’ve also gathered the best Labor Day laptop deals at Best Buy.The 2019 Vizio P Series Quantum is back under $999.Be sure to check out Amazon’s roughly three dozen Labor Day deals on TVs and audio. Google Express is having a big sale as well, one that includes deals on game consoles, AirPods, iPhones, laptops and more.Below I’ve rounded up a handful of individual items I consider to be the cream of the crop, followed by a handy reference guide to other Labor Day sales. Keep in mind, of course, that products may sell out at any time, even if the sale itself is still running. Note that CNET may get a share of revenue from the sale of the products featured on this page. Share your voice Spotify and most other streaming services rely on compressed audio, which robs the listener of full fidelity. Enter Tidal, the only “major” service that delivers lossless audio — meaning at least on par with CD quality, if not better. Want to see (er, hear) the difference for yourself? Grab this excellent extended trial while you can. It’s just $6 for three months, and it’s good for up to six listeners. Read the AirPods review Tags 7 DJI Osmo Action camera: $261 (save $89) Amazon
Bangalore, Jan 18 (ANI): India’s third-largest Information technology (IT) services exporter, Wipro Limited, registered a 27 percent rise in quarterly net profit, topping estimates, bolstered by rising demand from overseas clients. Wipro chief executive officer, TK Kurien, aimed for a constant growth rate of four and four and half percent.
Pooja RainaPR HandoutLife is all about not giving up on your dreams. One of India’s top bloggers Pooja Raina left her job after she gave birth to her second child after a gap of 7 years. After her child’s birth, Pooja did not work for few years as she wanted to see the growing up days of her toddler.Later, when she was ready to get back to work, she was not sure of what she exactly wanted to do. That is when her friends suggested that she can start blogging as she has a vast knowledge of beauty, fashion and lifestyle. That is exactly what Pooja Raina did and because of her blog called ‘Makeup and Body Blog’ she made it to the list of ‘Top 50 lifestyle bloggers’ in a span of just 2 years.About the same, Pooja Raina said that children and career are equally important tasks and one is responsible how the future shapes up for each. Pooja said that she keeps her posts authentic and makes sure to do something different so that people get to see unique content on her blog.Pooja Raina also said, “For me being into fashion and beauty is all about sharing my personal style with the world. Always remember that your style should reflect your individuality! Own your style, be proud of it, and embrace fashion as a way to express who you are and who you want to be.”At this stage, Pooja Raina feels like a strong and independent woman. She is happy that her profession gives her the liberty to create her own schedule and also time to be with her kids.
Rescuers sit in a boat on Lake Victoria on 22 September, 2018, during searches for victims a day after the ferry MV Nyerere capsized. Photo: AFPTanzania declared the whole nation was in mourning Sunday as the first dozen bodies were buried from a devastating ferry capsize on Lake Victoria that left people 224 dead.Prime Minister Kassim Majaliwa led “national funerals” on the island of Ukara, where the MV Nyerere had been coming in to dock on Thursday.He spoke of “great mourning by the whole nation” as the first coffins were placed in individual graves, many of the victims unidentified.The remainder of the dead were to be lain to rest later or taken away by families wishing for privates funerals.The prime minister said a memorial would be built on Ukara.Hopes had faded of finding any more survivors three days after the disaster, even after rescuers pulled out an engineer on Saturday who had holed up in an air pocket in the upturned vessel.But Majaliwa said divers would continue the grim search in the waters around the boat. The ferry would also be refloated.He updated the death toll to 126 women, 71 men, 17 girls and 10 boys. Just 41 people survived.- ‘Overloading’ blamed for tragedy -Transport Minister Isack Kamwelwe said 265 people had been on board the ferry, which had an official capacity of 100 or 101 passengers.The prime minister said initial investigations suggested overloading was one of the causes of the accident.”We have already arrested all those people in charge of operating and supervising the MV Nyerere. Questioning has begun,” he said.A broader commission of inquiry into the disaster would also be set up, Majaliwa added.One survivor was an engineer who shut himself into a “special room” with enough air for him to stay alive until he was found, said local lawmaker Joseph Mkundi.Transport minister Kamwelwe said on Saturday that 172 of the bodies had been identified by relatives.State television cited witnesses reporting that more than 200 people had boarded the ferry at Bugolora, a town on the larger Ukerewe Island. It was market day, which usually sees the vessel packed with people and goods.Witnesses told AFP that the ferry sank when passengers rushed to one side to disembark as it approached the dock. Others blamed the captain, saying he had made a brusque manoeuvre.- Grief and anger -Dozens of wooden coffins had lined the shore on Saturday, waiting to be seen by families as police and volunteers sought to keep hundreds of curious locals at bay.Aisha William came to collect the body of her husband. “He left on Tuesday around noon, but he never came home. I do not know how I am going to raise my two children,” she said.Ahmed Caleb, a 27-year-old trader, railed at a tragedy “which could have been prevented. I’ve lost my boss, friends, people I went to school with,” he sighed.The ageing vessel, whose hull and propellers were all that remained visible above water, was also carrying cargo, including sacks of maize, bananas and cement, when it capsized.Tanzanian President John Magufuli on Friday ordered the arrest of the ferry’s management and declared four days of national mourning.”It appears clear that the ferry was overloaded”, he said, adding that the government would cover the funeral expenses.With a surface area of 70,000 square kilometres (27,000 square miles), oval-shaped Lake Victoria is roughly the size of Ireland and is shared by Tanzania, Uganda and Kenya.It is not uncommon for ferries to capsize on the lake, and the number of fatalities is often high due to a shortage of life jackets and the fact that many local people cannot swim.In 1966, more than 800 people lost their lives on Lake Victoria when the MV Bukoba sank off the mainland town of Mwanza, according to the Red Cross.
Industries have been set up encroaching the river banks. the picture taken from the banks of Balu river near the Sultana Kamal Bridge in Demra area. Photo: Prothom AloA list of 42,423 persons has been drawn up by the National River Protection Commission, naming those who have encroached upon rivers all around the country. The list includes tradesmen who have set up small shops as well as industrialists who have set up huge factories on the rivers. The encroachers include well-known persons and politicians. The commission has submitted the list to the parliamentary standing committee of the shipping affairs ministry.The encroachers have not only taken over rivers, but canals and wetlands all over the country. The local administration has drawn up lists of these encroachers upon directives from the river protection commission.The commission has said that the list will be scrutinized further to ensure no encroacher is left out. After that, a crash programme will be taken up to remove all illegal structures from the rivers within a year. The commission, however, has said it does not have adequate funds for the drive.The river protection commission is yet to receive the list of river encroachers in 12 districts including Dhaka, Dinajpur, Panchagarh, Gaibandha, Kurigram, Jamalpur, Bagerhat, Mymensingh and Netrakona. The administration of many districts has not given complete lists as yet. The commission has urged the concerned district administrations to submit the lists immediately.The lists indicate that local persons have set up shops and houses on most of the rivers. And in areas near around the capital city, alongside the local people, big industries have also encroached upon the rivers Sitalakkhya, Turag and Balu. In the port city Chattogram, the river Karnaphuli and the Chaktai canal are entangled in encroachments.On 1 July the Supreme Court passed a verdict declaring river encroachment to be a criminal offence. Rivers were declared to be living beings and the river protection commission was given legal guardianship of the rivers.Chairman of the river protection commission, Mujibur Rahman Howladar, told Prothom Alo, for the first time a list has been reared of river, canal and wetland encroachers. The deputy commissioners in the various districts basically carried out this task. This is a continual process and the lists will be scrutinized further to check if there are any more encroachers.He said a dive has already begun to remove the encroachments. A one-year crash programme will be taken up and implemented by the district administrations. He has called upon the government for adequate funds for the purpose.Sitalakkhya swamped by sand traders and dockyardsA look through the list shows that the banks of Sitalakkhya have been taken over by a number of dockyards. These include the Saudia, Shefa, Saud Khan, Abul Hossain and Ibrahim dockyards among others.Then again, sand traders are dumping sand into Sitalkhhya and filling it. The sand traders include HI Traders, Masum Enterprise, Salek Enterprise, Biplob Enterprise, Beauty Enterprise and Jashim Enterprise. Each of these traders has filled around 4800 sq ft of the river near Rupganj.State minister for shipping Khalid Mahmud Chowdhury has told Prothom Alo, the shipping ministry has a task force to protect the rivers. It would look after the rivers in and around Dhaka, but now, upon directives from the prime minister, it would look into all major rivers or the country.He said that the district administration has been told to take action against the encroachers, but this would require adequate funds. However, eviction has already begun in some districts.Industries on Turag, BaluThe rivers Turag and Balu on the outskirts of the capital city have also been illegally encroached upon by large industries. Anontex, Zarina Textiles, Anwar Group and other industrial groups have filled parts of the rivers and set up their structures.Karnaphuli encroachmentsAt least 777 persons have encroached upon the River Karnaphuli. Most of this has been done in the name of Bastuhara Samity (association of the homeless), by the association’s president and members. They have erected structures along the river.And another 2112 persons have occupied parts of Karnaphuli and tributaries. They have set up industries, temples and so on.Commercially important to Chattogram, the Chaktai canal has also been illegally encroached upon by at least 51 persons.And 10 persons have illegally occupied the river Banshkhali in Cox’s Bazar, by setting up fish farms there. Another 48 persons have set up bamboo houses and plantations on 13 acres of the river area. Similarly the rivers Sangu, Loharia, Laukathi, Ichhamati and others all over the country are disappearing under the establishments being set up by these illegal occupiers.Speaking to Prothom Alo about river encroachment, chairman of Transparency International Bangladesh (TIB), Iftekharuzzaman, said that it is a positive move that for the first time a list of river encroachers has been drawn up. More importantly, the list has been handed over to the parliamentary standing committee. The government should evict the encroachers with no discrimination or bias and also take legal action against them.* This report appeared in the print edition of Prothom Alo and has been rewritten in English by Ayesha Kabir
News Share This! Tagschurch membership church membership decline Gallup homepage featured nones,You may also like Facebook Twitter Pinterest LinkedIn ReddIt Email,(RNS) — Most Americans still say they believe in God, but their involvement in organized religion continues to wane.A new Gallup report found that only half of Americans say they belong to a church or other religious body, down from 69% two decades earlier.Most of the decline is tied to the rise of the so-called “nones” — those who claim no religious affiliation. Gallup found that the share of Americans who claim a religious identity declined from 90% to 77% in recent decades.But even those who claim a faith tradition may not belong to a religious congregation or community, according to the report, which compared data from 1998-2000 to data from 2016-2018.At the turn of the century, Gallup said, 73% of religious Americans belonged to a house of worship. That’s dropped to 64% today.“The still-sizable proportion of religious Americans also contribute to declining church membership, as fewer in this group belong to a church than did so two decades ago,” the report states.Being part of a house of worship is no longer necessary for a growing number of religious Americans, said Tim Carney, author of “Alienated America.”“This data confirms what we’ve been seeing for decades: American life is becoming deinstitutionalized,” Carney said. “Americans are less likely to belong to anything. In America, historically, the thing most people have belonged to has been the church, and now more and more people are losing that.”Age and generational differences appear to play a role in whether Americans join a house of worship.“Just 42 percent of millennials are members of churches, on average,” according to the report. “By comparison, 20 years ago, 62 percent of members of Generation X belonged to a church, when they were about the same age as millennials are today.”The poll found that 68% of “traditionalists” — which Gallup identifies as those born before 1945 — are part of a church or other religious body. That percentage has declined from 78% two decades ago.Gallup’s study also found that 89% of traditionalists have a religious identity, compared with 68% of millennials, which it defines as those born between 1980 and 2000.“Not only are millennials less likely than older Americans to identify with a religion, but millennials who are religious are significantly less likely to belong to a church,” according to the report. “Fifty-seven percent of religious millennials belong to a church, compared with 65 percent or more in older generations.”Among other findings:Catholics (63%) are less likely to belong to a church than Protestants (67%).Nondenominational Christians (57%) are less likely to belong to a church than those tied to a specific denomination (70%).Mormons have among the highest affiliation with a church, at about 90%.Jewish membership in synagogues has remained steady at about 50%.Gallup’s new numbers are striking because they suggest a even lower level in religiosity than other recent data on religious affiliation.Last year’s General Social Survey, a poll conducted since 1972 by the National Opinion Research Center at the University of Chicago, found that those who reported that they affiliate with “no religion” and those that self-identify as evangelicals are the same size — about 23%.The difference is one of wording, explained Ryan Burge, a pastor and political science researcher. While the GSS looked at affiliation, which can be more cultural, Gallup asked about membership, which might suggest a more formal connection with a specific religious body.“A lot of people say they’re Catholic, for example, but they never go to Mass,” Burge said. “Or they went to college, moved away, and don’t attend a church in their new town. So if you ask if they’re a member of the Catholic Church, they’re more likely to say they’re not because they don’t actively attend.”Burge said the gap between the population that claims a religious affiliation and population that claims church membership shows that America is still “marginally attached” to civic religion.“The vast majority of us think we should believe in something, that we should have some religious affiliation,” he said. “They’re not afraid of the label of religion, they’re just reluctant to engage in the activity. That ties into the larger problem in America where people aren’t joining stuff as much as they used to.”That lines up with an overall lack of interest in belonging, according to Harvard University political scientist Robert Putnam, whose 2000 book, “Bowling Alone: The Collapse and Revival of American Community,” argued that Americans have been engaging less and less in communal social activities – bowling leagues, Rotary clubs, Boy Scouts, gardening clubs, book clubs and more — since the 1960s.Church attendance, Putnam told Religion News Service, has simply followed this downward trend.In fact, the data on the decline in church membership shares “almost exactly the same pattern of ups and downs” as engagement in secular civil society, he said.While the acceleration of the trend may partially be rooted in the increased politicization of religion, Putnam said, it’s likely much less about religion and spirituality and much more about a general disengagement with organized social activities.That gap has only partially been filled by the rise of online forms of community, researchers say, which has consequences not only for faith groups but for society at large.“This is not a good trend, even from a secular perspective,” Carney said. “People who attend church have more connections, more friends, more support in tough times, and more of a sense of purpose. Families that attend church are stronger. And among the working class, the data suggest that people who drop out of church aren’t joining anything else in its place.”Putnam, though, cautioned against focusing on just the rearview mirror.“Just because a trend is going in a direction, doesn’t mean it can’t reverse course,” Putnam said. “I actually think it’s possible that the millennials will lead a renewal of civil society. There’s a decent chance we’re on the verge of a major change in American society.”And religion, he said, could very well be a part of that.The Gallup study was based on telephone interviews of American adults from all 50 states and the District of Columbia. Trend data on membership came from surveys of 2,000 adults. The margin of error is plus or minus 3 percentage points.Results for 2016-2018 are based on interviews with 7,688 Americans. Results from 1998-2000 are based on interviews with 7,184 Americans. Both have a margin of error of plus or minus 1 percentage point.(This story has been updated.) By: Aysha Khan ayshabkhan DIY Faith • News Dinner church movement sets the table for food, faith and friendships August 29, 2019 By: Aysha Khan ayshabkhan Share This! By: Aysha Khan ayshabkhan By: Bob Smietana @bobsmietana Cancel replyYou must be logged in to post a comment.,Emperor performs ritual to report abdication to Shinto gods Buttigieg walks fine line in courting religious left August 29, 2019 Aysha Khan ayshabkhan Pete Buttigieg: Religious left is ‘stirring’ August 29, 2019 Share This! Share This! News About the authorView All Posts How a controversial Muslim figure helped ignite the controversy over Rep. Ilhan Omar� … Facebook Twitter Pinterest LinkedIn ReddIt Email By: Bob Smietana @bobsmietana Facebook Twitter Pinterest LinkedIn ReddIt Email,About the authorView All Posts Bob Smietana Bob Smietana is a veteran religion writer and editor-in-chief of Religion News Service. By: Bob Smietana @bobsmietana Aysha Khan Aysha Khan is a Boston-based journalist reporting on American Muslims and millennial faith for RNS. Her newsletter, Creeping Sharia, curates news coverage of Muslim communities in the U.S. Previously, she was the social media editor at RNS.,Add Comment Click here to post a comment Facebook Twitter Pinterest LinkedIn ReddIt Email Facebook Twitter Pinterest LinkedIn ReddIt Email Share This! Bob Smietana @bobsmietana
More information: Robert M. Yarrington et al. Nucleosomes inhibit target cleavage by CRISPR-Cas9 in vivo, Proceedings of the National Academy of Sciences (2018). DOI: 10.1073/pnas.1810062115AbstractGenome editing with CRISPR-Cas nucleases has been applied successfully to a wide range of cells and organisms. There is, however, considerable variation in the efficiency of cleavage and outcomes at different genomic targets, even within the same cell type. Some of this variability is likely due to the inherent quality of the interaction between the guide RNA and the target sequence, but some may also reflect the relative accessibility of the target. We investigated the influence of chromatin structure, particularly the presence or absence of nucleosomes, on cleavage by the Streptococcus pyogenes Cas9 protein. At multiple target sequences in two promoters in the yeast genome, we find that Cas9 cleavage is strongly inhibited when the DNA target is within a nucleosome. This inhibition is relieved when nucleosomes are depleted. Remarkably, the same is not true of zinc-finger nucleases (ZFNs), which cleave equally well at nucleosome-occupied and nucleosome-depleted sites. These results have implications for the choice of specific targets for genome editing, both in research and in clinical and other practical applications. A team of researchers at the University of Utah has found that nucleosomes can inhibit CRISPR/Cas9 cleavage efficiency. In their paper published in Proceedings of the National Academy of Sciences, the group describes testing the gene editing technique on yeast samples and what they found. Journal information: Proceedings of the National Academy of Sciences Illustration of Cas9 binding to DNA. The top figure shows two nucleosomes surrounding a nucleosome-free stretch of DNA. Hypothetical PAM sites for Cas9 targets in the central region and the right-hand nucleosome are shown in red. The lower figure shows Cas9 (with sgRNA) bound to the central target, with the PAM and flanking DNA held deep in the protein cleft. The PAM in the nucleosome could not be accessed without dissociating the DNA from the histone core. DNA backbones are blue; the RNA backbone is teal; DNA and RNA bases are white, except for the PAM; histones are green; Cas9 is purple. Credit: Janet Iwasa (University of Utah, Salt Lake City). Citation: Researchers show that nucleosomes can inhibit CRISPR-Cas9 cleavage efficiency (2018, September 11) retrieved 18 August 2019 from https://phys.org/news/2018-09-nucleosomes-inhibit-crispr-cas9-cleavage-efficiency.html © 2018 Phys.org The gene editing technique CRISPR-Cas 9 uses guide RNA to find and snip out segments of DNA—but what happens when the targeted segment is part of a nucleosome? Prior research has suggested that in such instances, it is likely that cleavage efficiency would suffer. In this new effort, the researchers have carried out an in vivo test of such instances and found that prior research results were correct—using CRISPR-Cas 9 on nucleosomes may not work very well.DNA strands are tiny, but really long—approximately six feet long if stretched out. Because of that, cells have mechanisms for packing DNA into a cell nucleus. That mechanism involves rolling the strands into bunches around a given protein. Such rolled bunches are known as nucleosomes. Logic suggests that a technique for editing a strand of DNA might encounter difficulty because of accessibility issues. Other researchers have considered the possibility of such problems, but have studied them in test tubes or did their work on known strands that were not part of nucleosomes. In this new effort, the researchers sought to find out once and for all if CRISPR-Cas9 would work just as well editing strands that are part of nucleosomes in living tissue as it does on strands that are not.The work involved CRISPR-Cas9 gene editing using different guide RNAs in living yeast, which allowed for editing different targets. Some of the targets were in nucleosomes while others were not. The researchers report that cleavage efficiency was much lower in nucleosomes than in non-nucleosome areas. But they also found something else—when testing the gene editing technique called zinc fingers the same way, they found no such difference. The group suggests that in the future, gene editing efforts should start with nucleosome position maps to improve efficiency. Explore further Two unrelated studies result in discovery of CRISPR-Cas12a inhibitors This document is subject to copyright. Apart from any fair dealing for the purpose of private study or research, no part may be reproduced without the written permission. The content is provided for information purposes only.
The Aam Aadmi Party (AAP) on Tuesday came out in support of the Delhi government, which has again invited Lieutenant Governor (LG) Najeeb Jung’s wrath over the appointment of six Bihar police officers in the Anti-Corruption Branch (ACB). The party leaders claimed that the ACB did not come under the Lieutenant Governor’s jurisdiction. Claiming to wage a tough war to weed out corruption from Delhi, party leaders alleged that those engaged in such malpractices were Also Read – Need to understand why law graduate’s natural choice is not legal profession: CJI